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pRL124
pRL124
規(guī)格:
貨期:
編號:B212101
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRL124
商品貨號 B212101
Designations pRL124
Depositors RE Loughlin
Biosafety Level 1
Vector Information
Size (kb): 6.2300000190734860
Vector: pRL124 (plasmid)
Promoters: Promoter none
Construction: pMC1403, pKO-100, M13 IG, trpA terminator, polylinker
Marker(s):ampR
Construct size (kb): 6.230000019073486
Features: insert detection: lacZ
marker(s): ampR
promoter: none
replicon: pMB1
terminator: trpA
Applications
promoter-cloning vector
vector containing primer sites useful for sequencing
vector permitting production of single-stranded DNA
vector permitting visual detection of activity in colonies
vector useful for in-vitro mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6, 0.3; EcoRI--6.2.
This is a promoter-cloning vector with beta-galactosidase reporter gene, trp a terminator upstream of MCS, galK leader with stop codons in 3 reading frames, and intergenic region of M13 to permit ssDNA preparation for mutagenesis and/or sequencing.
RL724 (ATCC 37683) and RL726 (ATCC 37684) differ in having the M13 intergenic region in opposite orientations in a different sized fragment.
This plasmid was constructed from the galK leader of pKO100 fused to the lacZ of pMC1403; the M13 intergenic region of pZ152; the multiple cloning sites of M13mp19; and the trp a terminator of pRL118.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Malo MS, Loughlin RE. Promoter-detection vectors for Escherichia coli with multiple useful features. Gene 64: 207-215, 1988. PubMed: 2841194

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