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pSE1112
pSE1112
規(guī)格:
貨期:
編號:B211691
品牌:Mingzhoubio

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RNA

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產(chǎn)品名稱 pSE1112
商品貨號 B211691
Designations pSE1112
Species Homo sapiens, human
Depositors SJ Elledge
Applications
in another host, produces protein SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAS1
Intact vector size: 7.100
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI
Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 DNA-binding domain, ->
epitope tag: hemagglutinin (HA), ->
restriction site: EcoRI, ->, 4315
MCS: NdeI...SalI, ->, 4395-4422
terminator: ADC1
replicon: pMB1
marker(s): ampR
replicon: 2 micron
marker(s): TRP1, ->
replicon: f1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: SNF1
Gene name: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.3
Cross references:
Insert lengths(kb): 2.299999952316284
Gene product: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase [SNF1]
Target Gene: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase
Insert Size (kb) 2.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; HindIII--5.8, 3.4; BamHI--7.0, 2.3.
pSE1111 (ATCC 87010) and pSE1112 (ATCC 87011) can be used together as a positive control in 2-hybrid cloning systems.
Expresses SNF1 fused to the GAL4 DNA-binding domain.
References

Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581

Stephen J Elledge, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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