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lambdaSP19
lambdaSP19
規(guī)格:
貨期:
編號(hào):B211388
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) lambdaSP19
商品貨號(hào) B211388
Designations lambdaSP19
Species Homo sapiens, human
Vector
Construct size (kb): 44.09999847412109
Insert
DNA: genomic
Insert lengths(kb): 14.60000038146973
Tissue: T-lymphocyte SP-F7 cell line
Gene product: T cell receptor, gamma, variable region V5 [TCRGC1]
Insert Size (kb) 14.600
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): SmaI--19.0, 13.0, 6.4, 4.6; BglII--27.0, 8.0, 7.5; EcoRI--23.0, 10.0, 3.4, 2.8, 2.5; BamHI--22.0, 20.0; HindIII--22.0, 11.5 (doublet); PvuI--20.0, 15.0, 8.5; SacI--20.0, 9.4, 8.5, 4.4.
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--1.65, 5.4, 5.8, 12.1; EcoRI--3.05, 6.0, 8.3, 11.0; HindIII--3.15, 3.6, 7.8, 12.5; BamHI--14.6.
The 3' end of this insert is the naturally occurring BamHI site in C1 exon 1.
Contains germline V5, germline V4, the 5' end of C1 exon 1, and an unusual rearrangement abutting the V3 recombination signal to J1 in an inverted orientation.
lambdaSP14 (ATCC 65836, 65837) and lambdaSP19 (ATCC 65838, 65839) are end clones from the same inversion (47 kb) event.
References

Alexandre D, et al. Reciprocal hybrid joints demonstrate successive V - J rearrangements on the same chromosome in the human TCR gamma locus. Int. Immunol. 3: 973-982, 1991. PubMed: 1836738

梅經(jīng)理 17280875617 1438578920
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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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