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pCu416CUP1
pCu416CUP1
規(guī)格:
貨期:
編號(hào):B211300
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCu416CUP1
商品貨號(hào) B211300
Designations pCu416CUP1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 5.3800001144409180
DESCRIPTION OF VECTOR:
Intact vector size: 5.380
Type of vector: phagemid
Cloning sites: SpeI BamHI SmaI EcoRI EcoRV ClaI SalI XhoI
Polylinker sites: SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI Xho
Construction: pRS416, CUP1 promoter
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->
replicon: f1, <-
terminator: CYC1, <-
MCS: XhoI...SpeI, ->
promoter for expression: CUP1, ->
replicon: pMB1
marker(s): ampR, <-
centromere: CEN6
replicon: ARSH4
Vector: pCu416CUP1 (phagemid)
Promoters: Promoter for expression CUP1
Construction: pRS416, CUP1 promoter
Marker(s):URA3,ampR
Construct size (kb): 5.380000114440918
Features: marker(s): URA3
marker(s): ampR
promoter for expression: CUP1
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6
Applications
cloning vector
Comments
Restiction digests of the vector give the following sizes (kb): SacI--5.4; SacI/SpeI--5.4 (has lost SpeI site).
Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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Shipping Information Distributed: DNA supplied by depositor (frozen) (amount: 100 ng)
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