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PTEN-P8
PTEN-P8
規(guī)格:
貨期:
編號:B210387
品牌:Mingzhoubio

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產(chǎn)品名稱 PTEN-P8
商品貨號 B210387
Organism Mus musculus, mouse
Tissue Prostate epithelium
Product Format frozen
Morphology Epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Prostate cancer tumor
Age 10 month
Gender Male
Applications PTEN signaling pathways and prostate cancer
Shipping Information frozen
Storage Conditions liquid nitrogen vapor phase
Karyotype Chromosome Count: near 6N (113-125)
Numerical Abnormalities: -1, -4, +10, +14, +15, +16, +17, -Y
Structural Abnormalities: Der(2)T(2;11), Del(7C), Del(8A3), Del(10A1), Del(13B), Del(14C), Del(15B)
Images ATCC CRL-3031 Cell Micrograph
Derivation Prostate cancer tissue dissected from 10-month, intact Pten.loxp/loxp;PB-Cre4+ mouse
Receptor Expression androgen receptor +
Comments PTEN mutations are one of the the most frequent genetic alterations found in human prostate cancers. This cell line, PTEN-P8, along with its isogenic partner, PTEN-CaP8 was generated to better understand the underlying molecular mechanisms of PTEN in prostate cancer progression and control, as well as the signaling pathways controlled by PTEN. PTEN-P8 is heterozygous for Pten deletion. Down-regulation of the Cre transgene produces very low to undetectable levels of Cre protein expression in this cell line. This cell line was generated from tissue that had not been subjected to hormone ablation therapy and are thus ideal for the study of human refractory prostate cancer formation, as many of the most well-studied human prostate cancer cell lines are from late-stage cancer tissue that has undergone such therapy.
Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco’s Modified Eagle’s Medium, (ATCC® No. 30-2002). To make the complete growth medium, add the following components to 500 ml of the base medium:

fetal bovine serum (FBS; ATCC® No. 30-2020) to a final concentration of 10%

25 µg/mL bovine pituitary extract (BPE)

5 µg/mL human recombinant insulin

6 ng/mL human recombinant epidermal growth factor (EGF)

 

Note: Do not filter complete medium
Subculturing S/C when culture reaches ~70-85% confluence.

A subcultivation ratio of 1:20 to 1:50 is recommended.


1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces
of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted
microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 8.0 X 103
to 1.0 x 104 viable cells/cm2 is recommended.
6. Incubate cultures at 37°C.

Renew medium every 2 to 3 days.
Culture Conditions Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Population Doubling Time approximately 14 hours
Name of Depositor Dr. Jing Jiao & Dr. Hong Wu
Year of Origin 2004
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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