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22D1
22D1
規(guī)格:
貨期:
編號(hào):B210349
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 22D1
商品貨號(hào) B210349
Organism Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma)
Cell Type hybridoma:lymphoblast B lymphocyte; somatic cell hybri
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is useful for FACS and immunocytochemistry assays.
The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays.
Comments
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) to 4 X 10(4) viable cells/ml.
Interval: Subculture when cells reach a concentration of 8 X 10(5) cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype hamster, Armenian IgG
Population Doubling Time 12 hours
Name of Depositor CG Park, RM Steinman
Year of Origin May, 2003
References

Kang YS, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc. Natl. Acad. Sci. USA 101: 215-220, 2004. PubMed: 14694198

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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