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Influenza A virus (H3N2)
Influenza A virus (H3N2)
規(guī)格:
貨期:
編號:B210327
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Influenza A virus (H3N2)
商品貨號 B210327
Classification Orthomyxoviridae, Alphainfluenzavirus
Deposited As Influenza A virus
Agent Influenza A virus (H3N2)
Strain A/Victoria/3/75
Applications
Influenza Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Hemagglutinin member of H3 subgroup, but antigenically variant from prior strains. Neuraminidase member of N2 subgroup, but antigenically variant from prior strains. Cross-reacts with A/Hong Kong/68, A/England/42/72, A/Port Chalmers/73 and A/Scotland/74 viruses.
Effect on Host
Hemagglutinates chicken red blood cells and possible death of chicken embryo
Recommended Host
10 day old incubated specific pathogen free embryonated chicken eggs
Growth Conditions Temperature: 33°C
Recommendations for Infection: Dilute virus with D-PBS prior to inoculation. While candling the eggs, draw a pencil line around the air sac. Using an 18.5 gauge needle, poke a hole into the air sac. Using a 1.0 mL syringe fitted with a 22.5 gauge needle, insert the needle through the hole and into the allantoic cavity. Inject 0.2 mL of diluted inoculum into the allantoic cavity. Seal the hole with nail polish or sterile tape. Incubate eggs at 33°C 2-3 days, checking embryo viability every 12-18 hours and discard non-viable eggs.
To harvest allantoic fluid, refrigerate eggs for at least 2 hour post incubation. Use sterile scissors to cut away the shell around the air sack. Aspirate the allantoic fluid with a syringe or a pipette. Avoid capturing blood or yolk in the allantoic fluid.
Incubation: 2-3 days with humidity
Name of Depositor AP Kendal
Source Isolated from humans, Melbourne, Australia
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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