| 產(chǎn)品名稱 |
pobIT [OBIT] |
| 商品貨號 |
B208894 |
| Designations |
pobIT [OBIT] |
| Species |
Mus musculus, mouse |
| Depositors |
ML Lane |
| Vector |
Construct size (kb): 5.199999809265137 |
| Insert |
DNA: genomic Insert lengths(kb): 1.200000047683716 Tissue: NIH 3T3 cells transformed by DNA from S49 lymphoma Gene product: T lymphoma oncogene [Tlm] |
| Insert Size (kb) |
1.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/AVAI--3.0, 2.5; HindIII--5.4; AvaI--5.4; BamHI--uncut; AccI--4.0, 1.6. pobIT contains 5' flanking sequences and is a single copy probe. This probe detects genomic BamHI fragments of 18 and 8.7 kb in normal NIH 3T3 cells and transformed cell lines. The 8.7 kb band corresponds to the clone. This probe detects genomic HindIII fragments of approximately 30 kb and 6.2 kb in normal 3T3 cells and transformed cell lines. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Lane MA, Tobin MB. Genomic sequence of the mouse oncogene tlm. Nucleic Acids Res. 18: 3410, 1990. PubMed: 2356136
Lane MA, et al. Isolation and characterization of a stage-specific transforming gene, Tlym-I, from T-cell lymphomas. Proc. Natl. Acad. Sci. USA 81: 2227-2231, 1984. PubMed: 6326114
|
