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pAB366 [pAmPh366]
pAB366 [pAmPh366]
規(guī)格:
貨期:
編號(hào):B223779
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pAB366 [pAmPh366]
商品貨號(hào) B223779
Designations pAB366 [pAmPh366]
Depositors BM Tyler
Biosafety Level 1
Vector Information
Size (kb): 4.1999998092651370
Vector: pAB366 (plasmid)
Construction: pRMH200, pAm200
Marker(s):ampR bleR
Construct size (kb): 4.199999809265137
Features: marker(s): ampR bleR
replicon: pMB1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.7, 1.5; BamHI/XhoI--3.4, 0.8; BglI--3.0, 1.2.
Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa.
Shuttle vector conferring bleomycin and phleomycin resistance.
The order of the major features in this plasmid is: pUC8 - EcoRI - BamHI - am promoter - ble - XhoI - am 3' region - HindIII - pUC8.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844

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梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
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