久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pFL59-
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pFL59-
pFL59-
規(guī)格:
貨期:
編號:B216827
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pFL59-
商品貨號 B216827
Designations pFL59-
Depositors F Lacroute
Biosafety Level 1
Vector Information
Size (kb): 4.8010001182556150
Vector: pFL59- (phagemid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):TRP1,ampR
Construct size (kb): 4.801000118255615
Features: insert detection: lacZ'
marker(s): ampR, TRP1
promoter: lac
replicon: pMB1, f1, ARS-CEN6
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.8; BglII--4.0, 0.85; ClaI--4.0, 0.8.
YC-type low copy number shuttle vector permitting visual detection of recombinants based on alpha complementation. Can be used to produce ssDNA.
The order of the major features in this plasmid is: EcoRI/MCS/HindIII - TRP1 - ARS-CEN6 - pMB1 ori - ampR - f1 ori.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Yanisch-Perron C, et al. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119, 1985. PubMed: 2985470

Bonneaud N, et al. A family of low and high copy replicative, integrative and single-stranded S. cerevisiae/E. coli shuttle vectors. Yeast 7: 609-615, 1991. PubMed: 1767589

Bach ML, et al. Evidence for transcriptional regulation of OMP-decarboxylase in yeast by hybridisation of mRNA to the yeast structural gene cloned in E. coli. Proc. Natl. Acad. Sci. USA 76: 386-390, 1979. PubMed: 370827

Dotto GP, et al. The functional origin of Bacteriophage f1 DNA replication. J. Mol. Biol. 172: 507-521, 1984. PubMed: 6607361

Panzeri L, Philippsen P. Centromeric DNA from chromosome VI in Saccharomyces cerevisiae. EMBO J. 1: 1605-1611, 1982. PubMed: 6327282

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
彭山县| 衡阳县| 获嘉县| 奉新县| 裕民县| 昭觉县| 平罗县| 五指山市| 安顺市| 泉州市| 庄浪县| 兰溪市| 大余县| 乌兰县| 阜南县| 郴州市| 古交市| 鹤峰县| 光泽县| 临颍县| 建宁县| 仁布县| 隆子县| 永春县| 鄄城县| 班玛县| 苍溪县| 浦县| 理塘县| 克山县| 大埔县| 荆门市| 达孜县| 瑞丽市| 红原县| 大渡口区| 池州市| 岢岚县| 涞水县| 凤庆县| 北流市|