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Aspergillus terreus Thom
Aspergillus terreus Thom
規(guī)格:
貨期:
編號:B166813
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Aspergillus terreus Thom
商品貨號 B166813
Strain Designations MF4845
Application
Produces hypocholesterolemic agents
Produces hypolipemic agents
Produces lovastatin
Produces mevinolin
Produces mevinolinic acid
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Preceptrol® no
Genome Sequenced Strain

Yes

Comments Genome sequencing strain (Microbia, Inc., USA)
Medium ATCC® Medium 200: YM agar or YM broth
ATCC® Medium 324: Malt extract agar
ATCC® Medium 336: Potato dextrose agar (PDA)
Growth Conditions
Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

GGAAGGATCATTACCGAGTGCGTGTCTTTATGGCCCAACCTCCCACCCGTGACTATTGTACCTTGTTGCTTCGGCGGGCCCGCCAGCGTTGCTGGCCGCCGGGGGGCGACTCGCCCCCGGGCCCGTGCCCGCCGGAGACCCCAACATGAACCCTGTTCTGAAAGCTTGCAGTCTGAGTGTGATTCTTTGCAATCAGTTAAAACTTTCAACAATGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCCCGGCTTGTGTGTTGGGCCCTCGTCCCCCGGCTCCCGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTCGTCTTCCGCTCCGTAGGCCCGGCCGGCGCCCGCCGACGCATTTATTTGCAACTTGTTTTTTTCCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA


D1D2 region of the 28S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAGAGCTCAAATTTGAAAGCTGGCTCCTTCGGGGTCCGCATTGTAATTTGCAGAGGATGCTTCGGGTGCAGCCCCCGTCTAAGTGCCCTGGAACGGGCCGTCATAGAGGGTGAGAATCCCGTATGGGGCGGGGTGTCTGCGTCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGTGGTAAATTTCATCTAAAGCTAAATACTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAACCAGACTCGCTCGCGGGGTTCAGCCGGGCTTCGGCCCGGTGTACTTCCCCGCGGGCGGGCCAGCGTCGGTTTGGGCGGCCGGTCAAAGGCCTCCGGAATGTAGCGCCCTTCGGGGCGCCTTATAGCCGGGGGTGCAATGCGGCCAGCCTGGACCGAGGAACGCGCTTCGGCACGGACGCTGGCATAATGGTTGTAAACGA


beta-tubulin gene

TGTGCCCGATCCCCCTGTTCCCCTCTCCCCCGACGCGTCTCTTTTGGGCTGCTCTCTGAAGTCCAACCCCACCCGTTCCTGGAGAAACTTTGGATGAATTGACTAACATTCTTTCTTCTTCGTGATCATAGGTTCATCTGCAAACCGGCCAGTGTGTAAGTGCGACCGTGTCCTTTCGATGAGGATGGGAATAGGATGTTTTGTGACAGAGACTGAACTGGGTGGTGGTGATAGGGTAACCAAATTGGTGCCGCTTTCTGGTACGTCTGGAATCAACCTGGGGAATGCTGGCTCTCGTGGGATGCAGAGTCTTACGGACATGCGTCCTCGGGCTTAAAAGGGTTCCGTGGTGGCATGATGCTGACAACTGTACAGGCAAACCATCTCTGGCGAGCACGGCCTTGATGGCTCCGGTGTGTAAGTGTCACCGACGCCCGCTCAATGGGCTCCCATAATAGAGGATTACATGACGATGGACGATTCTGATGTGGAAACAGCTTCAATGGCTCCTCCGACCTCCAGCTCGAGCGCATGAACGTCTACTTCAACGAGGTACGTCCCTTCCACACCATCCTGTGACAGATTCTCCACGCTTAAAAGACCTCGACACTAATTTCCGATCCCCTTTAGGCCAGCGGAAACAAGTATGTTCCTCGTGCTGTCCTCGTTGACCTTGAGCCCGGTACCATGGACGCCGTCCGTGCCGGTCCCTTCGGTCAGCTCTTCCGTCCCGACAACTTCGTCTTTGGCCAGTCTGGTGCCGGTAACAACTGG
Name of Depositor Merck & Co., Inc.
U.S. Patent Number
Cross References

Nucleotide (GenBank) : GU256759 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : AABT00000000 Aspergillus terreus ATCC 20542, whole genome shotgun sequencing project

References

Greenspan MD, Yudkovitz JB. Mevinolinic acid biosynthesis by Aspergillus terreus and its relationship to fatty acid biosynthesis. J. Bacteriol. 162: 704-707, 1985. PubMed: 3988710

Moore RN, et al. Biosynthesis of the hypocholesterolemic agent mevinolin by Aspergillus terreus. Determination of the origin of carbon, hydrogen, and oxygen atoms by 13C NMR and mass spectrometry. J. Am. Chem. Soc. 107: 3694-3701, 1985.

Lam TY. Hypocholesterolemic fermentation products. US Patent 4,376,863 dated Mar 15 1983

Hajko P, et al. Process for the isolation of lovastatin. US Patent 5,712,130 dated Jan 27 1998

Alberts AW, et al. Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent. Proc. Natl. Acad. Sci. USA 77: 3957-3961, 1980. PubMed: 6933445

Askenazi M, et al. Integrating transcriptional and metabolite profiles to direct the engineering of lovastatin-producing fungal strains. Nat. Biotechnol. 21: 150-156, 2003.

Schierova M, Linka M, Pazoutova S. Sulfate assimilation in Aspergillus terreus: analysis of genes encoding ATP-sulfurylase and PAPS-reductase. Curr. Genet. 38: 126-131, 2000. PubMed: 11057445

Hendrickson L, et al. Lovastatin biosynthesis in Aspergillus terreus: characterization of blocked mutants, enzyme activities and a multifunctional polyketide synthase gene. Chem. Biol. 6: 429-439, 1999. PubMed: 10381407

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周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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