| 產(chǎn)品名稱(chēng) |
TK6 |
| 商品貨號(hào) |
B165868 |
| Organism |
Homo sapiens, human |
| Tissue |
spleen |
| Cell Type |
lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hereditary spherocytosis |
| Age |
5 years |
| Gender |
male |
| Applications |
The cells are heterozygous at the thymidine kinase (TK) locus and can be used to quantitatively detect forward mutations at 3 loci (resistance to trifluorothymidine (tk locus)). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
This line is derivative of the WIL-2 cell line. |
| Clinical Data |
male |
| Comments |
Resistance to thioguanine (hprt locus), and resistance to ouabain (Na/K ATPase). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 105 and 1 X 106 cells/ml) |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 8,9.3 TPOX: 8,11 vWA: 17,20 |
| Name of Depositor |
Massachusetts Institute of Technology |
| Deposited As |
Homo sapiens |
| U.S. Patent Number |
|
| References |
Skopek TR, et al. Assay for mutagenesis in heterozygous diploid human lymphoblasts. US Patent 4,302,535 dated Nov 24 1981
Skopek TR, et al. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 84: 411-416, 1978. PubMed: 214074
Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231
Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642
|
