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SV-T2

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編號(hào):B165775

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產(chǎn)品名稱	SV-T2
商品貨號(hào)	B165775
Organism	Mus musculus, mouse
Cell Type	fibroblastSV40 transformed
Product Format	frozen
Morphology	fibroblast
Culture Properties	adherent
Biosafety Level	2 Cells contain polyomavirus DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age	14 to 17 day gestation embryo
Strain	BALB/c
Antigen Expression	H-2d
Genes Expressed	SV40 T-antigen,H-2d
Cellular Products	SV40 T-antigen
Tumorigenic	Yes
Effects	Yes, in BALB/c mice
Complete Growth Medium	Dulbecco's modified Eagle's medium, 90%; bovine calf serum, 10%
Subculturing	Protocol: Remove medium, add fresh 0.1% trypsin for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Twice per week
Name of Depositor	S Aaronson
Deposited As	Mus musculus
References	Aaronson SA, Todaro GJ. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J. Cell. Physiol. 72: 141-148, 1968. PubMed: 4301006 Aaronson SA, Todaro GJ. Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162: 1024-1026, 1968. PubMed: 4301647 Petrovic N, et al. Role of proximal promoter elements in regulation of renin gene transcription. J. Biol. Chem. 271: 22499-22505, 1996. PubMed: 8798416

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