| 產(chǎn)品名稱 |
MDBK (NBL-1) |
| 商品貨號(hào) |
B165130 |
| Organism |
Bos taurus, cow |
| Tissue |
kidney |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 [Cells contain bovine viral diarrhea virus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
adult |
| Gender |
male |
| Applications |
This cell line is suitable as a transfection host |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen. |
| Derivation |
The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby. The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145).- |
| Clinical Data |
male |
| Genes Expressed |
keratin |
| Virus Susceptibility |
Vesicular stomatitis, Orsay (Indiana)
Infectious bovine rhinotracheitis
Bovine parvovirus
Bovine adenovirus 2
Bovine adenovirus 3
Bovine viral diarrhea virus 1
,
Bovine viral diarrhea virus 1
Parainfluenza 3
|
| Virus Resistance |
poliovirus 2 |
| Comments |
A specific lot of CCL-22, MDBK (NBL-1) bovine kidney cells was found to be positive for bovine viral diarrhea virus (BVDV), following an investigation by ATCC (Dec. 2015). Testing for BVDV was performed in compliance with the Code of Federal Regulations, Title 9, Section 113.52 (E &F) using virus-specific fluorescent antibody (FA) technique as well as non-specific tests for hemadsorption and cytopathic effects (CPE). Samples were found to be positive for BVDV by FA. Hemadsorption and CPE were not observed in the sample inoculated cultures. As a result, the BSL status for CCL-22 was changed to “2”.
Subsequent lots of CCL-22, MDBK (NBL-1) bovine kidney cells are tested for BVDV and the results will be indicated on the Certificate of Analysis for the lot.
The cells are positive for keratin by immunofluorescence |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing |
Rinse the cell sheet twice with fresh 0.25% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
|
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
S Madin, NB Darby |
| Deposited As |
Bos taurus |
| Passage History |
The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982.
The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). |
| Year of Origin |
1957 |
| References |
Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776
Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321
Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528
USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001
|
| Cross References |
Nucleotide (GenBank) :
AF025996
Bos taurus supervillin mRNA, complete cds.
Nucleotide (GenBank) :
U64997
Bos taurus ribonuclease K6 gene, partial cds.
Nucleotide (GenBank) :
NC_002531
Alcelaphine herpesvirus 1, complete genome.
Nucleotide (GenBank) :
AJ238405
Bos taurus mRNA for elongation factor 1 alpha.
Nucleotide (GenBank) :
J05217
Bovine beta-1->4-galactosyltransferase mRNA, 5' end.
Nucleotide (GenBank) :
AF005370
Alcelaphine herpesvirus 1 L-DNA, complete sequence.
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