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LLC-WRC 256
LLC-WRC 256
規(guī)格:
貨期:
編號(hào):B164988
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 LLC-WRC 256
商品貨號(hào) B164988
Organism Rattus norvegicus, rat
Tissue mammary gland
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease mammary gland; Walker carcinoma
Strain Walker
Tumorigenic Yes
Effects
Yes, in Amsterdam rats
Virus Resistance
poliovirus 1
Complete Growth Medium Medium 199, 95%; horse serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor RN Hull
Deposited As Rattus norvegicus
References

American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956.

Hull RN. Establishing long-term cultures of mammalian normal, solid tumor, and ascites tumor cells on glass. Science 117:223, 1953. Pubmed : 13038481

Hull RN, Cherry WR, Johnson IS. The adaptation and maintenance of mammalian cells to continuous growth in tissue culture. Anat Rec 124: 490–499,1956.

Earle WR. A study of the Walker rat mammary carcinoma 256, in vivo and in vitro. Am J Cancer 24:566-612, 1935.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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