| 產(chǎn)品名稱 |
LL 47 (MaDo) |
| 商品貨號 |
B164979 |
| Organism |
Homo sapiens, human |
| Tissue |
lung |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
16 years |
| Gender |
male |
| Ethnicity |
Black |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
normal human male; diploid
At passage 9, the stemline number was diploid with a stable karyotype. One chromatid break was noted in 50 metaphase cells. The karyotype contained a brightly fluorescent Y chromosome as revealed by the quinacrine mustard fluorescence technique. Giemsa banding indicated no cross-contamination. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Derivation |
The line was established by K. Bradley from a biopsy from a normal area of the lung of a patient with osteogenic sarcoma |
| Clinical Data |
male
Black
16 years |
| Complete Growth Medium |
Ham's F12K medium, 85%; fetal bovine serum, 15%
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| STR Profile |
Amelogenin: X,Y CSF1PO: 7,12 D13S317: 9,11 D16S539: 11 D5S818: 12,13 D7S820: 8,10 THO1: 7,9.3 TPOX: 9,10 vWA: 14,16 |
| Isoenzymes |
G6PD, B |
| Name of Depositor |
RG Crystal |
| Deposited As |
Homo sapiens |
| Year of Origin |
November, 1975 |
| References |
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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