久久久久国产成人精品亚洲午夜,成人免费毛片一区二区三区,国产成人久久精品激情91,国产成人无码精品久久久免费,精品欧美国产一区二区三区不卡,国产成人一区二区三区影院,国产精品久久久久久,久久久WWW成人免费精品,欧美91精品久久久久网免费

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > DEC-205 [NLDC-145]
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
DEC-205 [NLDC-145]
DEC-205 [NLDC-145]
規(guī)格:
貨期:
編號:B164343
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 DEC-205 [NLDC-145]
商品貨號 B164343
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with mouse lymph node stroma.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen)
Comments

The antibody reacts with an antigen that is expressed at high levels on mouse dendritic cells and thymic cortical epithelial cells.

The antigen is a 205,000 dalton group VI C type molecule that mediates adsorptive endocytosis and antigen presentation.

The antibody reacts with and stains mouse dendritic cells and epidermal Langerhans cells.

The cells are capable of being adapted to serum free growth in using Nutridoma medium.

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 2 x 10and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2a
Name of Depositor RM Steinman, G Kraal
Deposited As rat (B cell); mouse (myeloma)
References

Kraal G, et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163: 981-997, 1986. PubMed: 3950549

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
聂荣县| 咸丰县| 祁门县| 沙坪坝区| 松阳县| 招远市| 永春县| 安化县| 修武县| 乌拉特后旗| 兴业县| 崇义县| 松潘县| 彭山县| 高州市| 奉化市| 英山县| 池州市| 鞍山市| 繁峙县| 安远县| 岑巩县| 安塞县| 静安区| 龙山县| 重庆市| 杭锦后旗| 辰溪县| 霸州市| 耿马| 石屏县| 灯塔市| 南平市| 东乡族自治县| 中宁县| 文登市| 长海县| 哈巴河县| 和硕县| 益阳市| 泸州市|