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A9
A9
規(guī)格:
貨期:
編號(hào):B163895
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 A9
商品貨號(hào) B163895
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Storage Conditions liquid nitrogen vapor phase
Derivation Cell membranes used for immunization derived from UT-1, a cell line derived from the CHO K1 cell line.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
Immunoglobulin; monoclonal antibody; against HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl Coenzyme A reductase, HMG CoA reductase)
Cellular Products
immunoglobulin; monoclonal antibody; against HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl Coenzyme A reductase, HMG CoA reductase)
Tumorigenic Yes
Effects
Yes, in pristane primed BALB/c mice
Comments

Animals were immunized with cell membranes from UT-1.

The antibody reacts with HMG-CoA reductase (the rate limiting enzyme in the cholesterol biosynthetic pathway).

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Isotype IgG1
Name of Depositor JL Goldstein, YK Ho
Deposited As mouse (B cell); mouse (myeloma)
References

Liscum L, et al. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe. J. Biol. Chem. 258: 8450-8455, 1983. PubMed: 6688077

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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