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Animals were immunized with human lymphocytes.

Spleen cells were fused with NS-1 myeloma cells.

The antibody has conventional CD45 reactivity and is included as antibody 815 in the panel used in the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens.

The antibody has a higher affinity for CD45 than the BC8 antibody (see ATCC HB-10507).

• fetal bovine serum to a final concentration of 10%
• 0.05 mM 2-mercaptoethanol

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶ cells/mL.

Matthews DC, et al. Radiation delivery to lymphoid and marrow tissues. US Patent 5,273,738 dated Dec 28 1993

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.