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8A3B.6
8A3B.6
規(guī)格:
貨期:
編號(hào):B163843
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) 8A3B.6
商品貨號(hào) B163843
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the structural protein VP7.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Cellular Products
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with bluetongue virus (serotype 17).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody is specific for the structural protein VP7.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 6 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 4 X 10 exp5 cells/ml.
Isotype IgG2a
Name of Depositor JA House
Deposited As mouse (B cell); mouse (myeloma)
References

Appleton JA, Letchworth GJ. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286-299, 1983. PubMed: 6186076

House C, et al. Detection of bluetongue group-specific antibody by competitive ELISA. J. Vet. Diagn. Invest. 2: 137-139, 1990. PubMed: 1965579

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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