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Vaccinia virus ts mutant
Vaccinia virus ts mutant
規(guī)格:
貨期:
編號:B163246
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Vaccinia virus ts mutant
商品貨號 B163246
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts19
Applications
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection.
Biosafety Level 2 BSL 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This conditional lethal mutant, originally designated plaque isolate no. 2096, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B, indicating that it induces foci of a fibrous viroplasmic matrix substance without membranes in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection. Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses
Product Format frozen
Storage Conditions -70°C or colder
Comments
This conditional lethal mutant, originally designated plaque isolate no. 2096, was isolated from the IHD-W strain of Vaccinia by S. Dale after mutagenesis with nitrosoguanidine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM category B, indicating that it induces foci of a fibrous viroplasmic matrix substance without membranes in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location E11, a 129 amino acid core protein that is synthesized late during infection. Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses
Effect on Host
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing
CPE: enlargement, plaques with rounding and eventual sloughing
Growth Medium ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
Recommended Host
Production Host: BSC-40 (ATCC CRL-2761)

Recommended Host: BSC-40 (ATCC CRL-2761)

Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2
Growth Conditions
Duration: 1-3 days at 31 C with 5% CO2
Name of Depositor S Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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